| 產品編號 | bs-12399R-PE |
| 英文名稱 | Rabbit Anti-Multicilin/PE Conjugated antibody |
| 中文名稱 | PE標記的多纖毛素蛋白抗體 |
| 別 名 | IDAS; Mci; MCI_HUMAN; Multicilin; Protein Idas. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領域 | 細胞生物 信號轉導 干細胞 細胞周期蛋白 轉錄調節(jié)因子 細胞分化 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Rat, (predicted: Human, Mouse, Dog, Horse, ) |
| 產品應用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 42kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Multicilin |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產品介紹 |
background: Transcription regulator required for multiciliate cell differentiation. Acts by promoting transcription of genes required for multiciliate cell formation. Probably acts in a multiprotein complex (By similarity). Plays a role in mitotic cell cycle progression by promoting cell cycle exit. Function: Multiciliate cells function prominently in the respiratory system, brain ependyma and female reproductive tract to produce vigorous fluid flow along epithelial surfaces. These specialized cells form during development when epithelial progenitors undergo an unusual form of ciliogenesis, in which they assemble and project hundreds of motile cilia. Notch inhibits multiciliate cell formation in diverse epithelia, but how progenitors overcome lateral inhibition and initiate multiciliate cell differentiation is unknown. Here we identify a coiled-coil protein, termed multicilin, which is regulated by Notch and highly expressed in developing epithelia where multiciliate cells form. Inhibiting multicilin function specifically blocks multiciliate cell formation in Xenopus skin and kidney, whereas ectopic expression induces the differentiation of multiciliate cells in ectopic locations. Multicilin localizes to the nucleus, where it directly activates the expression of genes required for multiciliate cell formation, including foxj1 and genes mediating centriole assembly. Multicilin is also necessary and sufficient to promote multiciliate cell differentiation in mouse airway epithelial cultures. These findings indicate that multicilin initiates multiciliate cell differentiation in diverse tissues, by coordinately promoting the transcriptional changes required for motile ciliogenesis and centriole assembly. Subunit: Homodimer. Interacts with GMNN; targets GMNN to the nucleus, prevents GMNN interaction with CDT1 and competes with IDAS homodimerization. Subcellular Location: Nucleus. Excluded from the nucleolus. DISEASE: Probable target of the anaphase promoting complex/cyclosome (APC/C) which regulates its level in the cell during the mitotic cell cycle. Highly expressed during interphase and early mitosis. Expression decreases during anaphase to become undetectable during telophase and cytokinesis. Similarity: Belongs to the geminin family. Database links: Entrez Gene: 345643 Human Omim: 614086 Human SwissProt: D6RGH6 Human Unigene: 394578 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 多纖毛素(multicilin)可以促進細胞長出許多纖毛。當細胞暴露在多纖毛素中，它們長出許多纖毛的遺傳機制被激活。在發(fā)育中的胚胎里，這種蛋白指導某種位于肺部、腎臟和皮膚表面的干細胞發(fā)育為多纖毛細胞。如慢性哮喘、肺氣腫和囊腫性纖維化(cystic fibrosis)之類呼吸道疾病的病人經常遭受肺部感染，這可能是由于將保護性粘液從氣管中移走的多纖毛細胞受損導致的。在未來，干細胞治療可能用新的纖毛細胞替換這些受損的細胞，但是科學家首先需要知道如何指導干細胞沿著發(fā)育途徑長成多纖毛細胞。 |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
