| 產(chǎn)品編號 | bs-6435R-BF488 |
| 英文名稱 | Rabbit Anti-KIRREL1/BF488 Conjugated antibody |
| 中文名稱 | BF488標(biāo)記的腎病樣蛋白1抗體 |
| 別 名 | Kin of IRRE like (Drosophila); Kin of IRRE like; Kin of IRRE like protein 1; Kin of IRRE-like protein 1; Kin of irregular chiasm like protein 1; Kin of irregular chiasm-like protein 1; KIRR1_HUMAN; Kirrel; MGC129542; MGC129543; NEPH 1; NEPH1; Nephrin like protein 1; Nephrin related; Nephrin-like protein 1; KIRR1_HUMAN. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 發(fā)育生物學(xué) 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | (predicted: Human, Mouse, Rat, ) |
| 產(chǎn)品應(yīng)用 | IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 82kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human KIRREL1/NEPH1 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: Neph1(Kirrel) belongs to a family of three closely related transmembrane proteins of the Ig superfamily with a structure similar to that of nephrin. Neph1 consists of a signal peptide, five Ig-like C2-type domains with the middle domain overlapping with a PKD-like domain, an RGD sequence, a transmembrane domain and a cytoplasmic tail. Neph1 is abundantly expressed in the kidney, specifically expressed in podocytes of kidney glomeruli, and plays a significant role in the normal development and function of the glomerular permeability. Neph1 interacts with nephrin in vitro and in vivo, and able to stimulate transcriptional activation in a model system, such as the activation the transcription factor AP-1 via the stimulation of a MAPK module. Neph1 is crucial for the integrity of the slit diaphragm, as Neph1 gene knockout mice results in effacement of glomerular podocytes, heavy proteinuria, and early postnatal death. . Tissue specificity: Abundantly expressed in kidney. Specifically expressed in podocytes of kidney glomeruli. Function: Plays a significant role in the normal development and function of the glomerular permeability. Signaling protein that needs the presence of TEC kinases to fully trans-activate the transcription factor AP-1 (By similarity). Subunit: Interacts with TJP1/ZO-1 and with NPHS2/podocin (via the C-terminus). Interacts with NPHS1/nephrin (via the Ig-like domains); this interaction is dependent on KIRREL glycosylation. Homodimer (via the Ig-like domains). Interacts when tyrosine-phosphorylated with GRB2 (By similarity). Subcellular Location: Cell membrane; Single-pass type I membrane protein. Tissue Specificity: Abundantly expressed in kidney. Specifically expressed in podocytes of kidney glomeruli. Post-translational modifications: Phosphorylation probably regulates the interaction with NSH2. Phosphorylated at Tyr-605 and Tyr-606 by FYN, leading to GRB2 binding (By similarity). N-glycosylated (By similarity). Similarity: Belongs to the immunoglobulin superfamily. Contains 5 Ig-like C2-type (immunoglobulin-like) domains. Database links: UniProtKB/Swiss-Prot: Q96J84.2 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
