| 產(chǎn)品編號 | bs-60081R |
| 英文名稱 | Histone H3 (tri methyl K4, serotonyl Q5) Rabbit pAb |
| 中文名稱 | |
| 產(chǎn)品類型 | 甲基化抗體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse (predicted: Rat) |
| 產(chǎn)品應用 | WB=1:500-1:2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG |
| 純化方法 | Antigen affinity purification |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | 組蛋白翻譯后修飾（PTM）是表觀遺傳學的關鍵機制，可調(diào)節(jié)染色質結構，稱為“組蛋白密碼”。血清素也稱為5-羥色胺（5-HT），是一種興奮性神經(jīng)遞質，可激活大腦中特定的膜受體來調(diào)節(jié)腦細胞的活動。在TGM2蛋白的催化下，5-羥色胺的氨基可與組蛋白H3第5位谷氨酰胺的側鏈羧基共價結合，形成組蛋白5-羥色胺（H3Q5ser）。H3Q5ser與相鄰的H3K4me3間相互影響。H3K4me3Q5ser可增強TFIID等轉錄因子與染色質的結合，從而激活發(fā)育中的嚙齒動物大腦或人類神經(jīng)元中下游基因的表達。 |
| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: lane 1: PTM-1420+H3K4me3/Q5ser peptides, lane 2: PTM-1420+unmodified peptides, lane 3: PTM-1420, 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: N2a
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 15 kDa
Observed MW: 15 kDa
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: HeLa, N2a, BRL, Mouse kidney
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 15 kDa
Observed MW: 15 kDa
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
