| 產(chǎn)品編號 | bsm-60464M |
| 英文名稱 | MTHFD1 Recombinant Mouse mAb |
| 中文名稱 | 亞甲基四氫葉酸脫氫酶單克隆抗體 |
| 別 名 | C1TC_HUMAN; C-1-tetrahydrofolate synthase, cytoplasmic; MTHFC; MTHFD; C1-THF synthase; Epididymis secretory sperm binding protein; C-1-tetrahydrofolate synthase, cytoplasmic, N-terminally processed; Methylenetetrahydrofolate dehydrogenase; methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1; CIMAH; |
| 研究領域 | 腫瘤 細胞生物 信號轉導 新陳代謝 |
| 抗體來源 | Mouse |
| 克隆類型 | Recombinant |
| 克 隆 號 | F9D9 |
| 交叉反應 | Human (predicted: Mouse) |
| 產(chǎn)品應用 | WB=1:500-1:1000,ICC/IF=1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 110 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG1/Kappa |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | PBS, Glycerol, BSA. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a protein that possesses three distinct enzymatic activities, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase and 10-formyltetrahydrofolate synthetase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. The trifunctional enzymatic activities are conferred by two major domains, an aminoterminal portion containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain. [provided by RefSeq, Jul 2008] Subcellular Location: Cytoplasm. Tissue Specificity: Ubiquitous. Database links: Entrez Gene: 4522 Human Entrez Gene: 108156 Mouse Omim: 172460 Human SwissProt: P11586 Human SwissProt: Q922D8 Mouse |
| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: room
temperature 2h
Secondary Ab: Goat Anti-Mouse IgG H&L
(HRP)
Lysate: 1: HeLa, 2: 293, 3: A20, 4: Raw264.7
Protein loading quantity: 20 μg
Exposure time: 10 s
Predicted MW: 102 kDa
Observed MW: 102 kDa
Cell line: HEK-293
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for bsm-60464M
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
