| 產(chǎn)品編號 | D-9409 |
| 英文名稱 | Alkaline Phosphatase(≥10U/mg) Protein |
| 中文名稱 | 堿性磷酸酶(來源于牛腸粘膜，進口) |
| 別 名 | Phosphatase, Alkaline from bovine intestinal mucosa; AP; ALP; Alkaline Phosphatase; Orthophosphoric-monoester phosphohydrolase (alkaline optimum); 堿性磷酸酶 |
| 克 隆 號 | |
| CAS | 9001-78-9 |
| 理論分子量 | kDa |
| 檢測分子量 | |
| 保存條件 | Store at -20 ℃. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品介紹 |
Alkaline phosphatase is highly purified from calf intestine. Alkaline phosphatase (ALP) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease. Typical uses in the lab for alkaline phosphatases include removing phosphate monoesters to prevent self-ligation, which is undesirable during plasmid DNA cloning. Another important use of alkaline phosphatase is as a label for enzyme immunoassays. 基本信息： CAS：9001-78-9 分子量：dimer ~160 kDa MDL：MFCD00131849 外觀：凍干粉末 來源：牛小腸黏膜 比活性：≥10 DEA units/mg solid 產(chǎn)品簡介： 堿性磷酸酶（ALP或AKP）是廣泛分布于人體肝臟、骨骼、腸、腎和胎盤等組織經(jīng)肝臟向膽外排出的一種酶。 在免疫學研究方面, 已廣泛應(yīng)用AP 標記抗體進行酶聯(lián)免疫熒光反應(yīng)(E LISA)和Western 印跡分析, 即將A P 與顯色劑或去磷酸化后能發(fā)光的底物相互作用來揭示靶與檢測酶復(fù)合物的存在, 與辣根過氧化物酶相比, AP 用作標記酶的優(yōu)點是穩(wěn)定性高、靈敏度高, 缺點是成本高、標記困難。在生物化學和分子生物學方面, 用AP 催化除去DNA 分子的5′末端磷酸基團以防止載體自連是基因克隆中的常規(guī)手段之一。 |
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
