| 產(chǎn)品編號 | C01-01011 |
| 英文名稱 | HEPES |
| 中文名稱 | 4-(2-羥乙基)-1-哌嗪乙磺酸（HEPES） |
| 別 名 | HEPES，F(xiàn)ree acid; 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid; hepesfreeacid; 4-(2-HYDROXYETHYL)-1-PIPERAZINEETHANESULFONIC ACID; 4-(2-HYDROXYETHYL)PIPERAZINE-1-ETHANESULFONIC ACID; 2-[4-(2-HYDROXYETHYL)-1-PIPERAZINE]ETHANESULFONIC ACID; 2-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]ETHANESULFONIC ACID;LABOTEST-BB LT00138109; LABOTEST-BB LT00233156 2-[4-(羥乙基)-1-哌嗪基]乙磺酸;N-(2-羥乙基)哌嗪-N'-乙烷磺酸;4-羥乙基哌嗪乙磺酸;N-(2-羥乙基)哌嗪-N'-2-乙烷磺酸;4-(2-羥乙基)-1-哌嗪乙磺酸;N-2-羥乙基哌嗪-N'-2-乙磺酸(HEPES);羥乙基哌嗪乙磺酸;2-[4-(2-羥乙基)-1-哌嗪乙烷]磺酸; 4-羥乙基哌嗪乙磺酸（HEPES）; |
| 克 隆 號 | |
| CAS | |
| 理論分子量 | kDa |
| 檢測分子量 | |
| 保存條件 | Store at RT. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品介紹 |
HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic organic chemical buffering agent. HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture.
CAS：7365-45-9 分子式：C8H18N2O4S 分子量：238.30 MDL：MFCD00006158 級別：BR，99% 純度：>99% 外觀/性狀：白色晶性粉末 用途：用物緩沖劑。Hepes是一種氫離子緩沖劑，能較長時間控制恒定的pH范圍，有效緩沖范圍為pH6.8-8.2?？捎糜谂渲频鞍滋崛【彌_液、細胞培養(yǎng)用緩沖液等，對細胞無毒性作用。使用終濃度為10-50mmol/L，一般培養(yǎng)液內(nèi)含20mmol/LHEPES即可達到緩沖能力，細胞培養(yǎng)常用10mmol/L。 |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
