| 產(chǎn)品編號 | bs-21704R |
| 英文名稱 | NEK6 Rabbit pAb |
| 中文名稱 | 高度相似的細(xì)胞周期蛋白激酶NEK6抗體 |
| 別 名 | Highly similar to cell cycle protein kinase CDC5/MSD2; NEK6; NEK6_HUMAN; Never in mitosis A-related kinase 6; NIMA(Never In Mitosis Gene A) Related Kinase 6; NimA related protein kinase 6; NimA-related protein kinase 6; Protein kinase SID6 1512; Protein kinase SID6-1512; Putative serine threonine protein kinase; Serine/threonine protein kinase Nek6; Serine/threonine-protein kinase Nek6; SID61512. |
| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 發(fā)育生物學(xué) 細(xì)胞周期蛋白 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Rat (predicted: Human,Mouse,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 36 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NEK6 : 81-180/313 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is a kinase required for progression through the metaphase portion of mitosis. Inhibition of the encoded protein can lead to apoptosis. This protein also can enhance tumorigenesis by suppressing tumor cell senescence. Several transcript variants encoding a few different isoforms have been found for this gene. Function: Protein kinase which plays an important role in mitotic cell cycle progression. Required for chromosome segregation at metaphase-anaphase transition, robust mitotic spindle formation and cytokinesis. Phosphorylates ATF4, CIR1, PTN, RAD26L, RBBP6, RPS7, RPS6KB1, TRIP4, STAT3 and histones H1 and H3. Phosphorylates KIF11 to promote mitotic spindle formation. Involved in G2/M phase cell cycle arrest induced by DNA damage. Inhibition of activity results in apoptosis. May contribute to tumorigenesis by suppressing p53/TP53-induced cancer cell senescence. Subunit: Interacts with STAT3 and RPS6KB1 (By similarity). Interacts with NEK9, predominantly in mitosis. Interacts with KIF11 (via C-terminus). Interacts with APBB1 (via WW domain). Interacts with ANKRA2, ATF4, ARHGAP33, CDC42, CIR1, PRAM1, PTN, PRDX3, PIN1, RAD26L, RBBP6, RPS7 and TRIP4. Subcellular Location: Cytoplasm. Nucleus. Nucleus speckle. Cytoplasm, cytoskeleton, centrosome. Cytoplasm, cytoskeleton, spindle pole. Note=Co-localizes with APBB1 at the nuclear speckles. Co-localizes with PIN1 in the nucleus. Co-localizes with ATF4, CIR1, ARHGAP33, ANKRA2, CDC42, NEK9, RAD26L, RBBP6, RPS7, TRIP4, RELB and PHF1 in the centrosome. Localizes to spindle microtubules in metaphase and anaphase and to the midbody during cytokinesis. [ALTERNATIVE PRODUCTS] Event=Alternative splicing; Named isoforms=4; Name=1; IsoId=Q9HC98-1; Sequence=Displayed; Name=2; IsoId=Q9HC98-2; Sequence=VSP_041798; Name=3; IsoId=Q9HC98-3; Sequence=VSP_041799; Name=4; IsoId=Q9HC98-4; Sequence=VSP_041800. Tissue Specificity: Ubiquitous, with highest expression in heart and skeletal muscle. Up-regulated in a variety of malignant cancers, such as breast, colon, lung, and gastric cancers. Post-translational modifications: Autophosphorylated. Phosphorylation at Ser-206 is required for its activation. Phosphorylated upon IR or UV-induced DNA damage. Phosphorylated by CHEK1 and CHEK2. Interaction with APBB1 down-regulates phosphorylation at Thr-210. Similarity: Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily. Contains 1 protein kinase domain. SWISS: Q9HC98 Gene ID: 10783 Database links: Clone ID: BC004174 Human Entrez Gene: 10783 Human Entrez Gene: 59126 Mouse IMAGE Id: 2958695 Human Omim: 604884 Human SwissProt: Q9HC98 Human SwissProt: Q9ES70 Mouse Unigene: 197071 Human Unigene: 143818 Mouse Unigene: 18744 Rat |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NEK6) Polyclonal Antibody, Unconjugated (bs-21704R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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