| 產(chǎn)品編號 | bsm-33113M |
| 英文名稱 | IL2RA/CD25 Mouse mAb |
| 中文名稱 | 白介素2受體a鏈/ICD25單克隆抗體 |
| 別 名 | Interleukin 2 receptor alpha chain; CD 25; CD25; CD25 antigen; IDDM10; IL 2 RA; IL 2 receptor alpha subunit; IL2 RA; IL-2RA; IL2R; IL2RA; IL2RAC; Interleukin 2 Receptor alpha; Interleukin 2 receptor alpha chain; Interleukin 2 receptor; Ly43; p55; T Cell Growth Factor Receptor; TAC Antigen; TCGFR; IL2RA_HUMAN. |
| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 細胞膜受體 t-淋巴細胞 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | 2B2 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | Flow-Cyt=1ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 28 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human IL2RA/CD25 |
| 亞 型 | IgG2a, κ |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009] Function: Receptor for interleukin-2. Subunit: Non-covalent dimer of an alpha and a beta subunit. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit). The high and intermediate affinity forms also associate with a gamma subunit. Subcellular Location: Membrane; Single-pass type I membrane protein. Similarity: Contains 2 Sushi (CCP/SCR) domains. Gene ID: 3559 Database links: Entrez Gene: 3559 Human Entrez Gene: 16184 Mouse SwissProt: P01589 Human SwissProt: P01590 Mouse Unigene: 231367 Human Unigene: 915 Mouse Omim: 147730 Human CD25抗原是分子量為55KD的單鏈糖蛋白，故又稱白介素2受體。主要用于與淋巴樣腫瘤、自身免疫性疾病及同種異體移植排斥反應(yīng)等疾病的研究。CD25是白介素受體的α亞單位，主要表達于活化的T細胞，在活化的B細胞、NK細胞和巨噬細胞也有一定量的表達。該抗體主要用于標記活化的淋巴細胞。 |
| 產(chǎn)品圖片 |
Blank control:SR.
Primary Antibody (green line): Mouse Anti-CD25 antibody (bsm-33113M)
Dilution: 1ug/Test;
Secondary Antibody (white blue line) : Goat anti-mouse IgG-FITC
Dilution: 0.5ug/Test.
Isotype control（orange line）：Normal Mouse IgG
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
scatter diagram showing peripheral blood lymphocytes stained with CD25. The cells were incubated with the antibody (bsm-33113M) for 30 min at 22°C.The secondary antibody used for 40 min at room temperature. Acquisition of >10,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
