| 產(chǎn)品編號 | bs-18527R |
| 英文名稱 | ZFP161 Rabbit pAb |
| 中文名稱 | 鋅指蛋白161抗體 |
| 別 名 | HZF5; MGC126126; ZBTB14; ZF161_HUMAN; ZF5; Zfp 161; Zfp-161; Zfp-5; Zfp161; Zinc finger and BTB domain containing protein 14; Zinc finger and BTB domain-containing protein 14; Zinc finger protein 161 homolog(mouse); Zinc finger protein 161 homolog; Zinc finger protein 478; Zinc finger protein 5; Zinc finger protein 5 homolog; Zinc finger protein homologous to Zfp161 in mouse; ZNF478. |
| 研究領(lǐng)域 | 神經(jīng)生物學 轉(zhuǎn)錄調(diào)節(jié)因子 鋅指蛋白 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Rat (predicted: Human,Mouse,Rabbit,Cow,Dog,Horse) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 51 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ZFP161: 181-280/449 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
ZFP161 is a 449 amino acid member of the Krüppel C2H2-type zinc-finger protein family. Localized to distinct regions within the nucleus, ZFP161 functions both as a transcriptional repressor of Thymidine Kinase (TK) and c-Myc promoters and as a transcriptional activator of the dopamine transporter (DAT) promoter. ZFP161 contains one BTB (POZ) domain and five C2H2-type zinc fingers which direct its specific localization and nucleic acid binding, respectively. Defects in the gene encoding ZFP161 may be associated with holoprosencephaly type 4 (HPE4), a structural anomaly of the brain characterized by a flattened nasal tip with no visible septum, hypotelorism, lack of nasal bridge, ptosis of the left upper eyelid and, in some cases, infant death. Function: Transcriptional repressor of MYC and thymidine kinase promoters (By similarity). Transcriptional activator of the dopamine transporter (DAT), binding it's promoter at the consensus sequence 5'-CCTGCACAGTTCACGGA-3'. Subcellular Location: Nucleus. Similarity: Belongs to the krueppel C2H2-type zinc-finger protein family. Contains 1 BTB (POZ) domain. Contains 5 C2H2-type zinc fingers. SWISS: O43829 Gene ID: 7541 Database links: Entrez Gene: 7541 Human Entrez Gene: 22666 Mouse Omim: 602126 Human SwissProt: O43829 Human SwissProt: Q08376 Mouse Unigene: 592340 Human |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZFP161) Polyclonal Antibody, Unconjugated (bs-18527R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
