| 產(chǎn)品編號 | bs-15510R |
| 英文名稱 | TYRP1 Rabbit pAb |
| 中文名稱 | 酪氨酸酶相關(guān)蛋白1抗體 |
| 別 名 | TRP; CAS2; CATB; GP75; OCA3; TRP1; TYRP; TRP-1; b-PROTEIN; tyrosinase related protein 1; TYRRP; 5, 6-dihydroxyindole-2-carboxylic acid oxidase; EC:1.14.18; Catalase B; Glycoprotein 75; Melanoma antigen gp75; Tyrosinase-related protein 1; TYRP1_HUMAN; |
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Specific References (4) | bs-15510R has been referenced in 4 publications.
[IF=7.971] Dvir Netanely. et al. Classification of node-positive melanomas into prognostic subgroups using keratin, immune, and melanogenesis expression patterns. Oncogene. 2021 Feb;:1-14 IHC ; Human.
[IF=5.395] Shubin Chong. et al. Silk Fibroin-Based Hydrogel Microneedles Deliver α-MSH to Promote Melanosome Delivery for Vitiligo Treatment. ACS BIOMATER-SCI ENG. 2023;XXXX(XXX):XXX-XXX WB ; Mouse.
[IF=1.65] Han, Joon‐Seung, Jong Hwan Sung, and Seung Kwon Lee. "Antimelanogenesis Activity of Hydrolyzed Ginseng Extract (GINST) via Inhibition of JNK Mitogen‐activated Protein Kinase in B16F10 Cells." Journal of Food Science (2016). WB ; Mouse.
[IF=1.35] Cui, Yucong, et al. "The expression of KRT2 and its effect on melanogenesis in alpaca skins." Acta histochemica (2016). WB ; Others.
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| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 染色質(zhì)和核信號 細(xì)胞類型標(biāo)志物 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat,Cow,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 58 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TYRP1: 101-200/537 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
TRP1 is a melanosomal enzyme that belongs to the tyrosinase family and plays an important role in the melanin biosynthetic pathway. Defects in this gene are the cause of rufous oculocutaneous albinism and oculocutaneous albinism type III. Function: Oxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5,6-quinone-2-carboxylic acid. May regulate or influence the type of melanin synthesized. Subcellular Location: Melanosome membrane; Single-pass type I membrane protein (By similarity). Note=Located to mature stage III and IV melanosomes and apposed endosomal tubular membranes. Transported to pigmented melanosomes by the BLOC-1 complex (By similarity). Tissue Specificity: Pigment cells. DISEASE: Albinism oculocutaneous 3 (OCA3) [MIM:203290]: An autosomal recessive disorder in which the biosynthesis of melanin pigment is reduced in skin, hair, and eyes. Tyrosinase activity is normal and patients have only moderate reduction of pigment. The eyes present red reflex on transillumination of the iris, dilution of color of iris, nystagmus and strabismus. Darker-skinned individuals have bright copper-red coloration of the skin and hair. Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Belongs to the tyrosinase family. SWISS: P17643 Gene ID: 7306 Database links: Entrez Gene: 7306 Human Entrez Gene: 22178 Mouse Omim: 115501 Human SwissProt: P17643 Human SwissProt: P07147 Mouse Unigene: 270279 Human Unigene: 30438 Mouse |
| 產(chǎn)品圖片 |
Sample: B16 (Mouse)cell Lysate at 40 ug
Primary: Anti-TRP1(bs-15510R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kD
Observed band size: 55 kD
Paraformaldehyde-fixed, paraffin embedded (Human skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Tyrosinase-related protein 1 ) Polyclonal Antibody, Unconjugated (bs-15510R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
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