| 產(chǎn)品編號 | bs-12280R |
| 英文名稱 | Stella Rabbit pAb |
| 中文名稱 | 發(fā)育多能性相關(guān)蛋白3抗體 |
| 別 名 | DPPA3; Compaction associated protein 1; Developmental pluripotency associated 3; Developmental pluripotency associated protein 3; Developmental pluripotency-associated protein 3; Dppa 3; Dppa3; DPPA3_HUMAN; PGC7; Stella related protein; Stella-related protein; Stellar. |
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Specific References (1) | bs-12280R has been referenced in 1 publications.
[IF=1.84] Wasielak, M., et al. "Zygote arrest 1 (ZAR-1), nucleoplasmin 2 (NPM2), and developmentally associated protein 3 (DPPA3) mRNA profiles throughout porcine embryo development in vitro." Theriogenology (2016). IHC-F ; Pig.
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| 研究領(lǐng)域 | 腫瘤 發(fā)育生物學 干細胞 轉(zhuǎn)錄調(diào)節(jié)因子 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Mouse (predicted: Human) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 18 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Stella/DPPA3: 101-159/159 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a protein that in mice may function as a maternal factor during the preimplantation stage of development. In mice, this gene may play a role in transcriptional repression, cell division, and maintenance of cell pluripotentiality. In humans, related intronless loci are located on chromosomes 14 and X. [provided by RefSeq, Jul 2008] Function: May play a role in maintaining cell pluripotentiality. Subcellular Location: Nucleus. Cytoplasm. Tissue Specificity: Low expression in testis, ovary and thymus. Expressed in embryonic stem and carcinoma cells. Highly expressed in testicular germ cell tumors. DISEASE: Expressed in fetal ovary. SWISS: Q6W0C5 Gene ID: 359787 Database links: Entrez Gene: 359787 Human Entrez Gene: 73708 Mouse Omim: 608408 Human SwissProt: Q6W0C5 Human SwissProt: Q8QZY3 Mouse Unigene: 131358 Human Unigene: 528118 Human Unigene: 27982 Mouse Unigene: 79056 Rat |
| 產(chǎn)品圖片 |
Sample:
Testis (Mouse) Lysate at 40 ug
Primary: Anti- Stella (bs-12280R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 18 kD
Observed band size: 18 kD
Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Stella/DPPA3 Polyclonal Antibody, Unconjugated(bs-12280R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
