| 產(chǎn)品編號 | bs-12245R |
| 英文名稱 | DAZL Rabbit pAb |
| 中文名稱 | 生殖細胞發(fā)育相關蛋白DAZL抗體 |
| 別 名 | DAZ homolog; DAZ like autosomal; DAZ-like autosomal; deleted in azoospermia-like; DAZH; DAZL 1; DAZL; DAZL_HUMAN; DAZL1; DAZLA; Deleted in azoospermia like 1; Deleted in azoospermia like; Deleted in azoospermia like autosomal; Deleted in azoospermia-like 1; Deleted in azoospermia-like; Germline specific RNA binding protein; MGC26406; Spermatogenesis gene on the Y like autosomal; SPGY like autosomal; SPGY-like-autosomal; SPGYLA; Tpx2. |
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Specific References (2) | bs-12245R has been referenced in 2 publications.
[IF=4.2] Zhang, Weidong, et al. "Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable." Chemosphere (2017). IHC-P ; Mouse.
[IF=2.638] Li T et al. Gene expression patterns and protein cellular localization suggest a novel role for DAZL in developing Tibetan sheep testes. Gene. 2020 Mar 20;731:144335. WB, IHC-P&IHF-P ; Tibetan sheep ram.
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| 研究領域 | 發(fā)育生物學 干細胞 轉錄調節(jié)因子 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Mouse (predicted: Human,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 33 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DAZL: 51-130/295 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The DAZ (Deleted in AZoospermia) gene family encodes potential RNA binding proteins that are expressed in prenatal and postnatal germ cells of males and females. The protein encoded by this gene is localized to the nucleus and cytoplasm of fetal germ cells and to the cytoplasm of developing oocytes. In the testis, this protein is localized to the nucleus of spermatogonia but relocates to the cytoplasm during meiosis where it persists in spermatids and spermatozoa. Transposition and amplification of this autosomal gene during primate evolution gave rise to the DAZ gene cluster on the Y chromosome. Mutations in this gene have been linked to severe spermatogenic failure and infertility in males. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]. Function: RNA-binding protein, which is essential for gametogenesis. Plays a central role during spermatogenesis. May act by binding to the 3'-UTR of mRNA and thereby regulating the translation of key transcripts. Subunit: Homodimer and heterodimer. Forms a heterodimer with DAZ. Interacts with BOLL, DAZAP1 and DAZAP2. Interacts with PUM2. Subcellular Location: Cytoplasm. Nucleus. Predominantly cytoplasmic. Nuclear in spermatogonia until near the end of the meiotic prophase and cytoplasmic localization from then onward. Tissue Specificity: Testis specific. Similarity: Belongs to the RRM DAZ family. Contains 1 DAZ-like domain. Contains 1 RRM (RNA recognition motif) domain. SWISS: Q92904 Gene ID: 1618 Database links: Entrez Gene: 1618 Human Entrez Gene: 13164 Mouse Omim: 601486 Human SwissProt: Q92904 Human SwissProt: Q64368 Mouse Unigene: 63695 Cow Unigene: 131179 Human Unigene: 280641 Mouse |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DAZL) Polyclonal Antibody, Unconjugated (bs-12245R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-Cy3) for 90 minutes, and DAPI for nuclei staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
