| 產(chǎn)品編號 | bs-0771R |
| 英文名稱 | CaMK2 alpha Rabbit pAb |
| 中文名稱 | 鈣/鈣調(diào)素依賴蛋白激酶2α抗體 |
| 別 名 | alpha CaMKII; CaMKII alpha; Calcium/Calmodulin Dependent Protein Kinase II G; Calcium calmodulin dependent protein kinase CaM kinase II alpha; Calcium calmodulin dependent protein kinase II alpha-B subunit; Calcium calmodulin dependent protein kinase II; Calcium/calmodulin dependent protein kinase II alpha; Calcium/calmodulin-dependent protein kinase(CaM kinase) II alpha; Calcium/calmodulin-dependent protein kinase II alpha; Calcium/calmodulin-dependent protein kinase II-alpha; Calcium/calmodulin-dependent protein kinase type II subunit alpha; Calcium/calmodulin-dependent protein kinase type IIA; CaM kinase II alpha chain; CaM kinase II alpha subunit; CaM kinase II beta chain; CaM kinase II beta subunit; CaM kinase II delta chain; CaM kinase II delta subunit; CaM kinase II subunit alpha; CAM2; CaMK II alpha subunit; CaMK II beta subunit; CaMK II delta subunit; CaMK-II subunit alpha; CAMK2A; CAMK2B; CAMK2D; CAMKA; CAMKB; CaMKII; CaMK 2a; CaMK2a; KCC2A_HUMAN. |
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Specific References (4) | bs-0771R has been referenced in 4 publications.
[IF=5.714] Ling Chen. et al. HC067047 as a potent TRPV4 inhibitor repairs endotoxemia colonic injury. INT IMMUNOPHARMACOL. 2023 Mar;116:109648 WB ; Mouse.
[IF=5.026] Di Xia et al. Nrf2 promotes esophageal squamous cell carcinoma (ESCC) resistance to radiotherapy through the CaMKIIα-associated activation of autophagy. Cell Biosci
. 2020 Jul 30;10:90. IHC ; Human.
[IF=3.414] Tang Q et al. Aqueous?extract?from?You-Gui-Yin?ameliorates?cognitive?impairment?of?chronic?renal?failure?micethrough?targeting?hippocampal?CaMKIIα/CREB/BDNF?and?EPO/EPOR pathways. J Ethnopharmacol. 2019 Jul 15;239:111925. WB ; Mouse.
[IF=3.414] Hou Y et al. Longzhibu disease and its therapeutic effects by traditional Tibetan medicine: Ershi-wei Chenxiang pills. J Ethnopharmacol. 2019 Nov 24:112426. IHC-P&WB ; Rat.
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| 研究領(lǐng)域 | 腫瘤 心血管 細(xì)胞生物 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) 激酶和磷酸酶 新陳代謝 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 54 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CaMK2 alpha: 101-200/478 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Ca2+/CalmodulinDependent Protein Kinase II (CaM Kinase II) is a multifunctional calcium and calmodulindependent protein kinase that mediates cellular responses to a wide variety of intercellular signals. CaM Kinase II has been shown to regulate diverse cellular functions including synaptic plasticity, neurotransmitter synthesis and release, gene expression, ion channel function, carbohydrate metabolism, cytoskeletal function, and Ca2+homeostasis. Phosphorylation of Thr286 on the kinase produces an autonomously active form of CaM Kinase II. Autophosphorylation of Thr305 inhibits the activity CaM Kinase II. Phosphorylation at this site appears to reduce the association of CaM Kinase II with the PSD and reduce LTP and learning. Function: CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. Subunit: CAMK2 is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. Interacts with BAALC, MPDZ, SYN1, CAMK2N2 and SYNGAP1 Subcellular Location: Cell junction, synapse, presynaptic cell membrane. Cell junction, synapse. Note=Postsynaptic lipid rafts. Similarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. Contains 1 protein kinase domain. SWISS: Q9UQM7 Gene ID: 818 Database links: Entrez Gene: 815 Human Entrez Gene: 816 Human Entrez Gene: 817 Human Entrez Gene: 818 Human Entrez Gene: 108058 Mouse Entrez Gene: 12322 Mouse Entrez Gene: 12323 Mouse Entrez Gene: 12325 Mouse Omim: 114078 Human SwissProt: Q13554 Human SwissProt: Q13555 Human SwissProt: Q13557 Human SwissProt: Q9UQM7 Human SwissProt: P11798 Mouse SwissProt: P28652 Mouse SwissProt: Q6PHZ2 Mouse SwissProt: Q923T9 Mouse Unigene: 725992 Human Unigene: 131530 Mouse Unigene: 107499 Rat |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with CaMK2 alpha polyclonal antibody, unconjugated (bs-0771R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Sample:
Lane 1: Mouse Heart tissue lysates
Lane 2: Rat Heart tissue lysates
Primary: Anti-CaMK2 alpha (bs-0771R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 50 kDa
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CaMK2 alpha) Polyclonal Antibody, Unconjugated (bs-0771R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
4% Paraformaldehyde-fixed SH-SY5Y (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (CaMK2 alpha) polyclonal Antibody, unconjugated (bs-0771R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
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