| 產(chǎn)品編號 | bs-6905R |
| 英文名稱 | CDCREL Rabbit pAb |
| 中文名稱 | 細胞周期調(diào)控相關(guān)蛋白1抗體 |
| 別 名 | 5 Sep; CDCrel-1; Cell division control related protein 1; Cell division control-related protein 1; H5 antibody HCDCREL 1; Peanut like 1; Peanut like 1 homolog; Peanut like protein 1; Peanut-like protein 1; Platelet glycoprotein Ib beta chain; PNUTL1; SEPT5; SEPT-5; SEPT 5; SEPT5_HUMAN; Septin 5; Septin5; Septin-5. |
| 研究領(lǐng)域 | 細胞生物 免疫學(xué) 細胞周期蛋白 細胞分化 細胞骨架 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Rat (predicted: Mouse,Pig,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 41 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CDCREL/SEPT5: 21-120/369 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced. [provided by RefSeq, Dec 2010]. Function: Filament-forming cytoskeletal GTPase. May play a role in cytokinesis (Potential). May play a role in platelet secretion. Subunit: Septins polymerize into heterooligomeric protein complexes that form filaments, and can associate with cellular membranes, actin filaments and microtubules. GTPase activity is required for filament formation. Interacts with SEPT2 and SEPT5. In platelets, associated with a complex containing STX4. Interacts with PARK2. This interaction leads to SEPT5 ubiquitination and degradation. Subcellular Location: Cytoplasm. Cytoplasm, cytoskeleton. Note=In platelets, found in areas surrounding alpha-granules Tissue Specificity: Expressed at high levels in the CNS, as well s in heart and platelets (at protein level). Post-translational modifications: In platelets, phosphorylated in response to thrombin, phorbol-12-myristate-13-acetate and collagen. Similarity: Belongs to the septin family. SWISS: Q99719 Gene ID: 5413 Database links: UniProtKB/Swiss-Prot: Q99719.1 SEPTIN(SEPT)家族是細胞骨架蛋白的一種，具有GTPase的活性。經(jīng)研究發(fā)現(xiàn)，SEPT參與細胞的分裂、細胞膜的運輸以及構(gòu)成細胞骨架。SEPTIN5又稱細胞骨架蛋白SEPT5。 |
| 產(chǎn)品圖片 |
Sample:
A549 Cell (Human) Lysate at 30 ug
Primary: Anti- CDCREL (bs-6905R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 46 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CDCREL) Polyclonal Antibody, Unconjugated (bs-6905R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CDCREL) Polyclonal Antibody, Unconjugated (bs-6905R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
