| 產(chǎn)品編號 | bs-5594R |
| 英文名稱 | phospho-VAV1 (Tyr174) Rabbit pAb |
| 中文名稱 | 磷酸化鳥苷酸轉(zhuǎn)換因子VAV1抗體 |
| 別 名 | VAV1(phospho Y174); Oncogene vav; p95Vav; Protooncogene vav; Proto-oncogene vav; VAV 1; VAV 1 oncogene; VAV; Vav proto oncogene; Vav proto-oncogene; VAV_HUMAN; VAV1. |
| 產(chǎn)品類型 | 磷酸化抗體 |
| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 染色質(zhì)和核信號 信號轉(zhuǎn)導(dǎo) 轉(zhuǎn)錄調(diào)節(jié)因子 激酶和磷酸酶 G蛋白偶聯(lián)受體 t-淋巴細胞 b-淋巴細胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Rat (predicted: Human,Mouse,Pig,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 98 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human VAV1 around the phosphorylation site of Tyr174: EI(p-Y)ED |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene is a member of the VAV gene family. The VAV proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. The encoded protein is important in hematopoiesis, playing a role in T-cell and B-cell development and activation. The encoded protein has been identified as the specific binding partner of Nef proteins from HIV-1. Coexpression and binding of these partners initiates profound morphological changes, cytoskeletal rearrangements and the JNK/SAPK signaling cascade, leading to increased levels of viral transcription and replication. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Apr 2012]. Function: Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation. Subunit: May interact with CCPG1. Interacts with APS, DOCK2, GRB2, GRB3, DOCK2, SLA, TEC and ZNF655/VIK. Interacts with SIAH2; without leading to its degradation. Associates with BLNK, PLCG1, GRB2 and NCK1 in a B-cell antigen receptor-dependent fashion. Interacts with CBLB; which inhibits tyrosine phosphorylation and down-regulates activity. Interacts with SHB and CLNK. Interacts with THEMIS2. Interacts with HCK. Interacts with NEK3 and this interaction is prolactin-dependent. Interacts with ITK. Interacts with PTK2B/PYK2. Interacts (via SH2 domain) with SYK. Interacts with ANKRD54. Tissue Specificity: Widely expressed in hematopoietic cells but not in other cell types. Post-translational modifications: Phosphorylated on tyrosine residues by HCK in response to IFNG and bacterial lipopolysaccharide (LPS). Phosphorylated by FYN. Similarity: Contains 1 CH (calponin-homology) domain. Contains 1 DH (DBL-homology) domain. Contains 1 PH domain. Contains 1 phorbol-ester/DAG-type zinc finger. Contains 1 SH2 domain. Contains 2 SH3 domains. SWISS: P15498 Gene ID: 7409 Database links: Entrez Gene: 7409 Human Entrez Gene: 22324 Mouse Omim: 164875 Human SwissProt: P15498 Human SwissProt: P27870 Mouse Unigene: 116237 Human Unigene: 248172 Mouse Unigene: 48861 Rat Vav1蛋白是特異于造血細胞表達的鳥苷酸轉(zhuǎn)換因子,它參與了多種免疫細胞如T細胞,B細胞,巨噬細胞,自然殺傷性細胞等的胞內(nèi)信號轉(zhuǎn)導(dǎo),發(fā)揮著重要作用. Vav1主要參與T細胞信號傳導(dǎo)途徑，傳導(dǎo)信號激活鈣流、細胞外信號調(diào)節(jié)激酶（ERK）以及轉(zhuǎn)錄因子NFκB。Vav1蛋白在T細胞生長發(fā)育、成熟T細胞激活、細胞因子合成及細胞骨架活動中發(fā)揮重要作用。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (phospho-VAV1 (Tyr174)) Polyclonal Antibody, Unconjugated (bs-5594R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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