| 產(chǎn)品編號 | bs-5046R |
| 英文名稱 | CYP46 Rabbit pAb |
| 中文名稱 | 膽固醇24S－羥化酶 |
| 別 名 | Cholesterol 24-hydroxylase; CP46; CYP46; CP46A_HUMAN; cytochrome P450 family 46. |
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Specific References (1) | bs-5046R has been referenced in 1 publications.
[IF=2.75] Meade et al. Quantitative proteomic analysis of the brainstem following lethal sarin exposure. (2015) Brain.Re. 1611:101-13 WB ; pig.
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| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) 新陳代謝 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Mouse (predicted: Human,Rat,Rabbit,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 57 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CYP46: 51-150/500 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The Cyp46 gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein is expressed exclusively in the brain, where it converts cholesterol to 24S-hydroxycholesterol by adding a hydroxyl group to cholesterol, producing a product that is more soluble than cholesterol and able to be exported from the brain. Cyp46 is also known as 24S-cholesterol hydroxylase. Function: Involved in the turnover of cholesterol. It converts cholesterol into 24S-hydroxycholesterol and, to a lesser extent, 25-hydroxycholesterol. Has also activity with xenobiotic compounds, such as clotrimazole. Subcellular Location: Endoplasmic reticulum membrane; Single-pass membrane protein. Microsome membrane; Single-pass membrane protein. Tissue Specificity: Expressed in brain. The mRNA was broadly distributed with higher levels in gray matter zones and lower levels in regions rich in white matter. Not detected in fetal sample but its expression increases linearly with age. Similarity: Belongs to the cytochrome P450 family. SWISS: Q9Y6A2 Gene ID: 10858 Database links: Entrez Gene: 10858 Human Entrez Gene: 13116 Mouse Omim: 604087 Human SwissProt: Q9Y6A2 Human SwissProt: Q9WVK8 Mouse Unigene: 25121 Human 經(jīng)研究發(fā)現(xiàn)CYP46和APOE-4基因都產(chǎn)生變異的人,患老年性癡呆癥的風(fēng)險(xiǎn)比正常人高將近10倍，尤其CYP46蛋白控制產(chǎn)生的一種酶,能幫助大腦分解多余的膽固醇;而APOE-4的基因在人體膽固醇的運(yùn)輸過程中起到作用。這說明膽固醇和β-淀粉蛋白的積聚之間也許存在某種關(guān)聯(lián),人體膽固醇新陳代謝的不足,也可能是導(dǎo)致老年性癡呆癥的“罪魁禍?zhǔn)住薄? |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CYP46) Polyclonal Antibody, Unconjugated (bs-5046R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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