| 產(chǎn)品編號 | bs-5006R |
| 英文名稱 | PGAM1 Rabbit pAb |
| 中文名稱 | 磷酸變位酶1抗體 |
| 別 名 | BPG dependent PGAM 1; BPG dependent PGAM1; BPG-dependent PGAM 1; OTTHUMP00000059414; PGAM 1; PGAM A; PGAM B; PGAM-B; PGAM1_HUMAN; PGAMA; PGAMB; phosphoglycerate mutase 1; phosphoglycerate mutase 1 brain; phosphoglycerate mutase A; phosphoglycerate mutase A nonmuscle form; phosphoglycerate mutase isozyme B. |
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Specific References (1) | bs-5006R has been referenced in 1 publications.
[IF=7.25] Yao, Chun, et al. "Role of FADD Phosphorylation in Regulating Glucose Homeostasis: from Proteomic Discovery to Physiological Validation." Molecular & Cellular Proteomics (2013). WB ; Mouse.
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| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 信號轉(zhuǎn)導(dǎo) 新陳代謝 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Mouse,Rat (predicted: Human,Pig,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 29 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞外基質(zhì) |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PGAM2: 101-200/254 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
PGAM1 belongs to the phosphoglycerate mutase family, BPG-dependent PGAM subfamily. It is involved in the interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Function: Interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase) and EC 3.1.3.13 (phosphatase), but with a reduced activity. Subunit: Homodimer. Tissue Specificity: In mammalian tissues there are two types of phosphoglycerate mutase isozymes: type-M in muscles and type-B in other tissues. Post-translational modifications: Acetylated at Lys-253, Lys-253 and Lys-254 under high glucose condition. Acetylation increases catalytic activity. Under glucose restriction SIRT1 levels dramatically increase and it deacetylates the enzyme. Similarity: Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily. SWISS: P18669 Gene ID: 5223 Database links: Entrez Gene: 5223 Human Entrez Gene: 18648 Mouse Omim: 172250 Human SwissProt: P18669 Human SwissProt: Q9DBJ1 Mouse Unigene: 592599 Human |
| 產(chǎn)品圖片 |
Sample:
Stomach (Mouse) Lysate at 40 ug
Primary: Anti-PGAM1 (bs-5006R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 29 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PGAM1) Polyclonal Antibody, Unconjugated (bs-5006R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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