| 產(chǎn)品編號 | bs-3958R |
| 英文名稱 | NDUFA8 Rabbit pAb |
| 中文名稱 | NADH脫氫酶α家族8抗體 |
| 別 名 | Complex I 19kD; CI 19KD; CI PGIV; Complex I 19k; Complex I PGIV; MGC793; NADH dehydrogenase(ubiquinone) 1 alpha subcomplex 8(19kD PGIV); NADH dehydrogenase(ubiquinone) 1 alpha subcomplex 8 19kDa; NADH dehydrogenase(ubiquinone) 1 alpha subcomplex 8; NADH d |
| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 信號轉(zhuǎn)導(dǎo) 轉(zhuǎn)錄調(diào)節(jié)因子 線粒體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse (predicted: Human,Rat,Pig,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 19 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 線粒體 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NDUFA8: 101-172/172 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
NDUFA8 is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Mammalian complex I is composed of 45 different subunits and transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Function: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Subunit: Complex I is composed of 45 different subunits. Subcellular Location: Mitochondrion. Mitochondrion intermembrane space. Post-translational modifications: May contain intrachain disulfide bonds, as evidenced by its electrophoretic mobility under reducing vs non-reducing conditions. Similarity: Belongs to the complex I NDUFA8 subunit family. Contains 2 CHCH domains. SWISS: P51970 Gene ID: 4702 Database links: Entrez Gene: 4702 Human Entrez Gene: 68375 Mouse Omim: 603359 Human SwissProt: P51970 Human SwissProt: Q9DCJ5 Mouse Unigene: 495039 Human Unigene: 19834 Mouse |
| 產(chǎn)品圖片 |
Tissue/cell: mouse brain tissue 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-NDUFA8 Polyclonal Antibody, Unconjugated(bs-3958R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
|
