| 產(chǎn)品編號 | bs-3529R |
| 英文名稱 | ID1 Rabbit pAb |
| 中文名稱 | DNA結(jié)合抑制因子1抗體 |
| 別 名 | bHLHb24; Class B basic helix-loop-helix protein 24; DNA binding protein inhibitor ID 1; DNA binding protein inhibitor ID1; DNA-binding protein inhibitor ID-1; Dominant negative helix loop helix protein; ID 1; ID; ID1; ID1_HUMAN; Inhibitor of Differentiation 1; Inhibitor of DNA binding 1. |
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Specific References (1) | bs-3529R has been referenced in 1 publications.
[IF=0.181] Wang S et al. Insulin-like growth factor-1 inhibits the expression of autophagic ID1 in cerebrovascular endothelial cells. Int J Clin Exp Med 2019;12(8):10486-10493. WB ; Human.
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| 研究領域 | 腫瘤 細胞生物 染色質(zhì)和核信號 信號轉(zhuǎn)導 轉(zhuǎn)錄調(diào)節(jié)因子 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Dog) |
| 產(chǎn)品應用 | Flow-Cyt=1ug/Test,ICC/IF=1:100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 16 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ID1: 51-155/155 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
ID proteins contain a helix-loop-helix (HLH) motif and regulate tissue-specific transcription within several cell lineages. They do not bind DNA directly, but inhibit lineage commitment by binding basic helix-loop-helix (bHLH) transcription factors through their HLH motif. ID proteins contribute to cell growth, senescence, differentiation and angiogenesis. Id1 mRNA is highly expressed in heart, lung and kidney and has lower expression in brain and liver. Two transcript variants encoding different isoforms have been found for this gene. Function: ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. Subunit: Heterodimer with other HLH proteins. Interacts with COPS5. Subcellular Location: Cytoplasm. Nucleus. Similarity: Contains 1 bHLH (basic helix-loop-helix) domain. SWISS: P41134 Gene ID: 3397 Database links: Entrez Gene: 3397 Human Entrez Gene: 15901 Mouse Omim: 600349 Human SwissProt: P41134 Human SwissProt: P20067 Mouse Unigene: 504609 Human Unigene: 444 Mouse Unigene: 2113 Rat |
| 產(chǎn)品圖片 |
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (ID1) polyclonal Antibody, Unconjugated (bs-3529R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (ID1) polyclonal Antibody, Unconjugated (bs-3529R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Blank control (blue line): A549 (fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice).
Primary Antibody (green line): Rabbit Anti-ID1 antibody (bs-3529R),Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1μg /test.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
