| 產(chǎn)品編號 | bsm-2026M |
| 英文名稱 | CGA Mouse mAb |
| 中文名稱 | 人絨毛膜促性腺激素α 亞基單抗 |
| 別 名 | GLHA_HUMAN; Glycoprotein hormones alpha chain; Anterior pituitary glycoprotein hormones common subunit alpha; HCG; LHA; FSHA; GPA1; GPHa; TSHA; GPHA1; Choriogonadotropin alpha chain; Chorionic gonadotrophin subunit alpha(CG-alpha); Follicle-stimulating ho |
| 研究領域 | 腫瘤 免疫學 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | 4A8 |
| 交叉反應 | Human |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 10 kDa |
| 檢測分子量 | |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Natural human hCG protein |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M PBS (pH7.4) with 0.02% Proclin300. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The four human glycoprotein hormones chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH) are dimers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits of these hormones are identical, however, their beta chains are unique and confer biological specificity. The protein encoded by this gene is the alpha subunit and belongs to the glycoprotein hormones alpha chain family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011] Function: Stimulates the ovaries to synthesize the steroids that are essential for the maintenance of pregnancy. Subunit: Heterodimer of a common alpha chain and a unique beta chain which confers biological specificity to thyrotropin, lutropin, follitropin and gonadotropin. Subcellular Location: Secreted. Similarity: Belongs to the glycoprotein hormones subunit alpha family. SWISS: P01215 Gene ID: 1081 Database links: Entrez Gene: 1081 Human SwissProt: P01215 Human HCG是由胎盤合體滋養(yǎng)層細胞產(chǎn)生的一種糖蛋白激素，主要用于胎盤滋養(yǎng)細胞和生殖細胞腫瘤如葡萄胎、絨癌的診斷和輔助診斷。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded Human pituitary gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CGA Monoclonal Antibody, Unconjugated(bsm-2026M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Mouse, sp-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CGA Monoclonal Antibody, Unconjugated(bsm-2026M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
(Negative control) Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CGA Monoclonal Antibody, Unconjugated(bsm-2026M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Mouse, sp-0024) and DAB (C-0010) staining.
Measured by its binding ability in a indirect ELISA. Immobilized Human HCG alpha protein, HisTag (Cat. bs-43085P) at 2 μg/mL (100 μL/well) can bind Mouse Anti-Human HCG alpha Antibody, the EC50 is 40.93 ng/mL.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
